themes_champagnat

Genes and proteins

Dynamics of gene expression in neurons and glia during physiological responses

Pascual O, Denavit-Saubié M, Dumas S, Kietzmann T, Gilhini G, Mallet J,Pequignot JM. Selective cardiorespiratory and catecholaminergic areas express the hypoxia-inducible factor-1_ (HIF-1_) under in vivo hypoxia in rat brainstem, 2001, Eur J Neurosci. 14 : 1981-1991.

Dynamics of gene expression in progenitors during development

Jungbluth, S., Willeke, K. and Champagnat, J. Segment-specific expression of connexin31 in the embryonic hindbrain is regulated by Krox-20, 2002, Developmental Dynamics, 223: 544-551.

Respiratory patterning by developmental genes

Borday C., Wrobel L., Fortin G., Champagnat J., Thaëron-Antôno C. and Thoby-Brisson M., Developmental Gene Control of Brainstem Function: Views from the Embryo, 2004, Progress in Biophysics and Molecular Biology, 84: 89-106.

Segmental genes

Jacquin, TD, Borday, V., Schneider-Maunoury, S., Topilko, P., Kato, F., Ghilini, G., Charnay, P. and Champagnat, J., Reorganization of pontine rhythmogenic neuronal network in Krox-20 knockout mice, Neuron, 1996, 17 : 747-758.

Neurotrophins

Erickson, JT, Conover, JC, Borday, V, Champagnat, J, Katz, DM, Mice lacking BDNF exhibit visceral sensory neuron losses distinct from mice lacking NT4 and display a severe developmental deficit in control of breathing, The Journal of Neuroscience, 1996, 16 : 5361-5371.

Cells

Single cell PCR multiplex to identify neurons in the generator in vitro

Thoby-Brisson, M, Cauli, B, Champagnat J, Fortin G and Katz, DM, Expression of functional TrkB receptors by rhythmically active respiratory neurons in the pre-Bötzinger complex of neonatal mice, 2003, The Journal of Neuroscience, 23: 7685-7689.

Rhythmic patterns to identify neurons in the generator in vitro

Rekling, JC, Champagnat, J and Denavit-Saubié, M, Electroresponsive properties and membrane potential trajectories of three types of inspiratory neurons in the newborn mouse brain stem in vitro. J. Neurophysiol. (1996) 75: 795-810.

Electrophysiological/pharmacological identification of membranes (single channels)

Jacquin, T.D., Descoins, C., Champagnat, J. and Denavit-Saubié, M. K+-channels of expiratory neurones in the brainstem preparation of the newborn rat. NeuroReport, (1997) 8 : 3673-3678.

Pharmacology and sub-cellular imaging

Morin-Surun MP, Collin T, Denavit-Saubié M, Baulieu EE, Monnet FP. Intracellular sigma1 receptor modulates phospholipase C and protein kinase C activities in the brainstem. Proc Natl Acad Sci U S A. (1999) 96: 8196-8199.

Circuits

Anatomo-functional identification of mutation-induced circuits in vivo & in vitro

Dominguez Del Toro, E., Borday, V., Davenne, M., Neun, R., Rijli, F., Champagnat, J. Generation of a novel functional neuronal circuit in Hoxa1 mutant mice. 2001, The Journal of Neuroscience, 21: 5637-5642.

Electrophysiological / pharmacological analysis of circuits in vitro (slice preparation)

Pascual O, Morin-Surun M-P, Barna B, Denavit-saubié M, Pequignot J-M, Champagnat J, Progesterone reverses the neuronal responses to hypoxia in rat nucleus tractus solitarius in vitro, 2002, Journal of Physiology, 544: 511-520.

Pharmacological identification of rhythmic circuits in vivo (microiontophoresis)

Haji, A., Pierrefiche, O., Takeda, R., Foutz, A.S., Champagnat, J., and Denavit-Saubié, M., Membrane potential of respiratory neurons during dizocilpine-induced apneusis in adult cats, J. Physiol. (Lond.), 1996, 495 : 851-861

Synaptic plasticity in neuronal circuits

Poon, C.S., Zhou, Z. and Champagnat, J. NMDA Receptor activity in utero averts respiratory depression and anomalous LTD in newborns, 2000 , The Journal of Neuroscience, 20: RC73: 1-6.

Behaviour

Functional compensations in the mutant respiratory network

Chatonnet F, Boudinot E, Chatonnet A, Taysse L, Daulon S, Champagnat J, Foutz AS, Respiratory survival mechanisms in acetylcholinesterase knockout mouse, 2003, European Journal of Neuroscience, 18: 1419-1427.

Non-lethal non-compensated respiratory phenotypes

Chatonnet, F., Dominguez del Toro, E, Voilescu, O., Charnay, P. Champagnat, J. Different respiratory control systems are affected in homozygous and heterozygous kreisler mutant mice, 2002, European Journal of Neuroscience 15: 684-692.

Breathing behaviour : plethysmographic recordings

Boudinot E, Tremblay P, Champagnat J, Foutz AS. Respiratory function in mice lacking or overexpressing the prion protein. Neurosci Lett 2002 26: 89-92.

Breathing behaviour : chemosensitivity

Boudinot E, Tamada M, Wess J, Champagnat J, Foutz, AS Ventilatory pattern and chemosensitivity in M1 and M3 muscarinic receptor knockout mice, 2004, Resp. Physiol. Neurobiol., 139: 237-245.

Embryonic specification of post-natal behavioural traits

Chatonnet F, Domínguez del Toro E, Thoby-Brisson M, Champagnat J, Fortin G, Rijli FM, Thaëron-Antôno C., From hindbrain segmentation to breathing after birth, developmental patterning in rhombomeres 3 and 4, Molecular Neurobiology 2003 29: 277-293.

Historical overview of current interest :

1984: Respiratory depression by opioids in vivo:

Morin-Surun, M.P., Gacel, G., Champagnat, J., Denavit-Saubié, M. and Roques, B., Pharmacological identification of delta and mu opiate receptors on bulbar respiratory neurons, Eur. J. Pharmacol., 1984, 98 : 241-247.

1984: circuit isolated in vitro:

Champagnat, J., Denavit-Saubié, M. and Siggins, G.R., Rhythmic neuronal activities in the nucleus of the tractus solitarius isolated in vitro, Brain Research, 1983, 280 : 155-159

1988: pharmacological identification of inspiratory offswitch:

Foutz, A.S., Champagnat, J., and Denavit-Saubié, M. N-methyl-D-aspartate (NMDA) receptors control respiratory off-switch in the cat. Neuroscience Letters, 1988, 87 : 221-226.

1993: glial control of neurons in vivo:

Ballanyi, K., Branchereau, P., Champagnat, J., Fortin, G. and Velluti, J.C., Extracellular potassium, glial and neuronal potentials in the solitary complex of rat brainstem slices, Brain Research, 1993, 607: 99-107.

1993: Intracellular pharmacology in vivo:

Champagnat, J. and Richter, D.W., Second messengers-induced modulation of the excitability of respiratory neurones, NeuroReport, 1993, 4 : 861-863.

1994: circuits in embryos: the segmented neural tube

Fortin, G., Champagnat, J. and Lumsden, A. Onset and maturation of branchio-motor activities in the chick hindbrain, NeuroReport, 1994, 5: 1149-1152.

1996: specification of neonatal breathing patterns by segmental genes:

1999: molecular mechanisms of rhythm formation in embryo:

Fortin, G. Jungbluth, S. Lumsden, A. and Champagnat, J. Segmental specification of GABAergic inhibition during development of hindbrain neural networks. 1999, nature neuroscience 2 : 873-877.